other:Article Title: Abnormal glycosylation changes in brain tissue of kainic acid-induced epileptic rats.
Article Snippet: Biotinylated lectins, including Aleuria aurantia lectin (AAL, 1:5000, Vector Laboratories), Lens culinaris agglutinin (LCA, 1:5000, Vector Laboratories), Sambucus nigra agglutinin (SNA, 1:10000, Vector Laboratories), Maackia amurensis lectin (MAL, 1:3000, Vector Laboratories), Phaseolus vulgaris Erythroagglutinin (PHA-E, 1:3000, Vector Laboratories), Phaseolus vulgaris Leucoagglutinin (PHA-L, 1:3000, Vector Laboratories) and Concanavalin A (ConA, 1:10000, Vector Laboratories) were purchased from Vector Laboratories Inc. (Burlingame, CA, USA) AR TIC LE IN PR ES S (Table 1).
Article Title: Abnormal glycosylation changes in brain tissue of kainic acid-induced epileptic rats
Article Snippet: Biotinylated lectins, including Aleuria aurantia lectin (AAL, 1:5000, Vector Laboratories), Lens culinaris agglutinin (LCA, 1:5000, Vector Laboratories), Sambucus nigra agglutinin (SNA, 1:10000, Vector Laboratories), Maackia amurensis lectin (MAL, 1:3000, Vector Laboratories), Phaseolus vulgaris Erythroagglutinin (PHA-E, 1:3000, Vector Laboratories), Phaseolus vulgaris Leucoagglutinin (PHA-L, 1:3000, Vector Laboratories) and Concanavalin A (ConA, 1:10000, Vector Laboratories) were purchased from Vector Laboratories Inc. (Burlingame, CA, USA) (Table ).
Staining:Article Title: MGAT1 knockout in human dendritic cells enhance CD8 + T cell activation
Article Snippet: .. Briefly, cells were stained with LIVE/DEADTM Fixable Yellow Dead Cell Stain to assess viability, before incubation with a selection of biotinylated lectins from Vector Laboratories: Aleuria aurantia lectin (AAL), Maackia amurensis lectin I and II (MAL-I and MAL-II), Sambucus nigra agglutinin (SNA), Phaseolus vulgaris lectin L (PHA-L) and Peanut agglutinin (PNA). .. Detection was achieved using AF488-conjugated streptavidin (Invitrogen), and both lectins and streptavidin were incubated with the cells on ice for 20 min.
Article Title: Evidence of an allostatic response by intestinal tissues following induction of joint inflammation
Article Snippet: Sagittal sections from knee joints were stained with Hematoxylin/Eosin, anti-CD68 (Abcam, Ab283654 ; 1:100) or anti-CD4 Abcam, Ab237722 1:2000). .. Sections of intestinal Swiss rolls were stained using the Alcian Blue Kit (Vector Labs, H-3501), anti-CD68 (1:100), anti-CD4 (1:2000), anti-CD8 (Abcam, Ab237709 , 1:500) or anti-Ki67 (Invitrogen, MA5–14520; 1:100), Invitrogen, MA5–14520) or the following biotinylated lectins (all from Vector Labs), Griffonia Bandeiraea Simplidifolia Lectin I, (GSL I, B-1105–2), Maackia Amurensis Lectin II (MAL II, B-1265–1) and Ulex Europaeus Agglutinin I (UEA, B-1065–2). .. Prior to histochemical staining, antigen retrieval was performed using Tris/EDTA (Abcam, ab93684), and sections were blocked with normal goat serum (Vector Laboratories, S-1000).
Article Title: Transgenic chicken that expresses a fluorescent protein and SIAT1
Article Snippet: .. Cells at 80-90% confluence were incubated with biotinylated lectins (Vector Laboratories, MAL II lectin for staining α2,3 residues, SNA lectin for staining α2,6 residues), then incubated with streptavidin-phycoerythrin conjugate, and then fixed in 4% paraformaldehyde for imaging under fluorescence. ..
Incubation:Article Title: MGAT1 knockout in human dendritic cells enhance CD8 + T cell activation
Article Snippet: .. Briefly, cells were stained with LIVE/DEADTM Fixable Yellow Dead Cell Stain to assess viability, before incubation with a selection of biotinylated lectins from Vector Laboratories: Aleuria aurantia lectin (AAL), Maackia amurensis lectin I and II (MAL-I and MAL-II), Sambucus nigra agglutinin (SNA), Phaseolus vulgaris lectin L (PHA-L) and Peanut agglutinin (PNA). .. Detection was achieved using AF488-conjugated streptavidin (Invitrogen), and both lectins and streptavidin were incubated with the cells on ice for 20 min.
Article Title: Transgenic chicken that expresses a fluorescent protein and SIAT1
Article Snippet: .. Cells at 80-90% confluence were incubated with biotinylated lectins (Vector Laboratories, MAL II lectin for staining α2,3 residues, SNA lectin for staining α2,6 residues), then incubated with streptavidin-phycoerythrin conjugate, and then fixed in 4% paraformaldehyde for imaging under fluorescence. ..
Selection:Article Title: MGAT1 knockout in human dendritic cells enhance CD8 + T cell activation
Article Snippet: .. Briefly, cells were stained with LIVE/DEADTM Fixable Yellow Dead Cell Stain to assess viability, before incubation with a selection of biotinylated lectins from Vector Laboratories: Aleuria aurantia lectin (AAL), Maackia amurensis lectin I and II (MAL-I and MAL-II), Sambucus nigra agglutinin (SNA), Phaseolus vulgaris lectin L (PHA-L) and Peanut agglutinin (PNA). .. Detection was achieved using AF488-conjugated streptavidin (Invitrogen), and both lectins and streptavidin were incubated with the cells on ice for 20 min.
Microscopy:Article Title: Method of isolating and detecting cancer stem cells
Article Snippet: .. Equipment used: Paraffin blocks, Ice, Microtome, Microtome, Superfrost® blades, Bond Max Automate (Leica Microsystems) with computer, Leica consumables (alcohol, wash buffer, ER1 buffer, dewax buffer, labels, coverslips, tubes), PBS-BSA buffer 5%, Biotinylated Lectins (UEA-1, Jacaline, ABA, ACA, GSL-I and GSL-II) (Vector Lab), Kit Bond Intense R detection (Leica), Leica mounting medium, slides and microscope. ..
Imaging:Article Title: Transgenic chicken that expresses a fluorescent protein and SIAT1
Article Snippet: .. Cells at 80-90% confluence were incubated with biotinylated lectins (Vector Laboratories, MAL II lectin for staining α2,3 residues, SNA lectin for staining α2,6 residues), then incubated with streptavidin-phycoerythrin conjugate, and then fixed in 4% paraformaldehyde for imaging under fluorescence. ..
Fluorescence:Article Title: Transgenic chicken that expresses a fluorescent protein and SIAT1
Article Snippet: .. Cells at 80-90% confluence were incubated with biotinylated lectins (Vector Laboratories, MAL II lectin for staining α2,3 residues, SNA lectin for staining α2,6 residues), then incubated with streptavidin-phycoerythrin conjugate, and then fixed in 4% paraformaldehyde for imaging under fluorescence. ..
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